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Journal Article

Laboratory based Publications

1Thapa J, Maharjan B, Malla M, Fukushima Y, Poudel A, Pandey BS, Hyashida K, Gordon SV, Nakajima C, Suzuki Y. Direct detection of Mycobacterium tuberculosis in clinical samples by a dry methyl green loop-mediated isothermal amplification (LAMP) method. Tuberculosis. 2019Download
2Paudel S, Nakajima C, Mikota SM, Gairhe KP, Maharjan B, Subedi S, Poudel A, Sashika M, Shimozuru M, Suzuki Y, and Tsubota T. Mixed Mycobacterium tuberculosis Lineage Infection in 2 Elephants, Nepal. Emerg Infect Dis 2019Download
3Ghimire S, Maharjan B, Jongedijk EM, Kosterink JGW, Ghimire GR, Touw DJ, Werf TSVD, Shrestha B, Alffenaar JWC. Levofloxacin pharmacokinetics and pharmacodynamics and outcome in MDR-TB patients. European Respiratory journal 2019.Download
4Ghimire S, Maharjan B, Jongedijk EM, Kosterink JGW, Ghimire GR, Touw DJ, Werf TSVD, Shrestha B, Alffenaar JWC. Evaluation of saliva as a potential alternative sampling matrix for therapeutic drug monitoring of levofloxacin in MDR-TB patients. Antimicrobial Agents Chemotherapy 2018Download
5Paudel S, Mikota SM, Thapa J, Lyashchenko KP, Gairhe KP, Dhakal IP, Subedi N, Maharjan B, Subedi S, Kaul GE, Tsubota T. Serodiagnosis of elephant tuberculosis: a useful tool for early identification of infected elephants at the captive-wild interface. European Journal of Wildlife Research 2018Download
6Maharjan B, NakajimaC, Isoda N, Thapa J, Poudel A, Shah Y, Yamaguchi T, Shrestha B, Hoffmann H, Avsar A, Shrestha A, Gordon SV & Suzuki S. Genetic diversity and distribution dynamics of multidrug-resistant Mycobacterium tuberculosis isolates in Nepal. Nature Scientific Report 2018Download
7Shrestha P, Shakya M, Caws M, Shrestha S, Karki B, Shrestha S, Karki DB, Maharjan B, Shrestha B, Arjyal A ,Karkey A, Prajapati KG, Basnyat B. Tuberculosis in Staff and Students of Patan Hospital. J Nepal Health Res Council 2018Download
8Poudel A, Maharjan B, Suzuki Y, Nakajima C. Predominance of modern sub lineage of Mycobacterium tuberculosis Beijing genotype among clinical isolates in Nepal. Int J Tuberc Lung Dis Vol 21 Nov 2017Download
9Shah Y, Maharjan B, Thapa J, Poudel A, Diab HM, Pandey BD, Solo ES, Isoda N, Suzuki Y, Nakajima C. High diversity of multidrug resistant Mycobacterium tuberculosis Central Asian Strain isolates in Nepal. Int J Infect Dis Jun 2017Download
10Thapa J, Nakajima C, Gairhe KP, Maharjan B, Paudel S, Shah Y, Mikota S, Kaufman GE , McCauley D, Tsubota T, Gordon SV and Suzuki Y. Wildlife Tuberculosis: An Emerging Threat for Conservation in South Asia. Book chapter: Global Exposition of Wildlife Management 2017Download
11Pandey P, Pant ND, Rijal KR, Shrestha B, Kattel S, Banjara MR, Maharjan B, KC R. Diagnostic Accuracy of GeneXpert MTB/RIF Assay in Comparison to Conventional Drug Susceptibility Testing Method for the Diagnosis of Multidrug-Resistant Tuberculosis. PLoS ONE. 2017Download
12Maharjan B, Kelly-Cirino CD, Weirich A, CurryPS , Hoffman H, Avsar K, Shrestha B. Evaluation of OMNIgene sputum-stabilized sputum for long term transport and Xpert MTB/RIF testing in Nepal. Int J Tubercle Lung Dis.2016Download
13Maharjan B, Shrestha B, Weirih A, Stewart A, Kelly-Cirino CD. A novel sputum transport solution eliminates cold chain and supports routine tuberculosis testing in Nepal. J Epidemiol Glob Health. 2016 Dec;6(4):257-265Download
14Thapa J, Paudel S, Sadaula A, Shah Y, Maharjan B, Kaufman G E....Nakajima C. Mycobacterium Origis-Associated Tuberculosis in Free-Ranging Rhinoceros, Nepal. Emerg Infect Dis. 2016 Mar;22(3):570-2.Download
15Simkhada RC, Shah Y, Thapa J, Adhikari N, Paudyal R, Khadka KS, Dahal B, Chhetri A, Amatya J, Maharjan B and Shrestha B. Study of Anti-Tuberculosis Drug Resistant Pattern among the Tuberculosis Patients Visiting German Nepal Tuberculosis Project. Donnish Journal of Infectious Disease and Immunity2016Download
16Thapa J, Nakajima C, Maharjan B, Poudel A, Suzuki Y. Molecular characterization of Mycobacterium orygis isolates from wild animals of Nepal. Jpn J Vet Res 94:287-292, 2015.Download
17Bhatt CP, Timalsina B, Kutu B, Pradhan R, Maharjan B, Shrestha B. A comparative of laboratory diagnostic methods of tuberculosis and aetiology of suspected cases of pulmonary tuberculosis. SAARC journal of Tuberculosis, Lung Disease & HIV/AIDS. 2015Download
18Bhatt CP, Timalsina B, Kutu B, Pradhan R, Maharjan B, Shrestha B. A comparative study on Ziehl-Neelsen staining (light microscopy), Auramine O staining (iLed-fluorescence microscopy) and Culture on L-J media of sputum samples for the diagnosis of pulmonary tuberculosis. International Journal of Medicine & Biomedical Sciences. 2015Download
19Sharma BK, Bhandari S, Maharjan B, Shrestha B, & Banjara MR. Rapid Detection of Rifampicin and Isoniazide resistant Mycobacterium tuberculosis Using Genotype MDBDRplus Assay in Nepal. Hindawi. 2014Download
20Tharu MB, Harries AD, Goel S, Srivastav S, Kumar AMV, Adhikari M, Shrestha B, Maharjan B, Khadka H. Screening retreatment tuberculosis patients for drug resistance in mid-west Nepal: how well are we doing? Public Health Action. 2014Download
21 Khadka JB, Maharjan B, Bhatta DR, Ghimire G. Early diagnosis of MDR-TB cases directly on sputum specimens by rapid molecular method. International journal of Microbiology Research and ReviewsVol 1 P 92-96 July 2013Download
22Paudel S, Mikota SK, Nakajima C, Gairhe KP, Maharjan B, Thapa J, Poudel A, Shimozuru M, Suzuki S, Tsubota T. Molecular characterization of Mycobacterium tuberculosis isolates from elephants of Nepal. Tuberculosis.2013Download
23Nakajima C, Tamaru A, Rahim Z, Poudel A, Maharjan B, Aye KS, Ling H, Hattori T, Iwamoto T, Fukushima Y, Suzuki H, Suzuki Y, and Matsuba T. Simple Multiplex PCR Assay for Identification of Beijing Family Mycobacterium tuberculosis Isolates with a Lineage-Specific Mutation in Rv0679c. J Clin Microbiol. 2013Download
24Poudel A, Maharjan B, Nakajima C, Fukushima Y, Pandey BD, Beneke A, Suzuki Y. Characterization of extensively drug-resistant Mycobacterium tuberculosis in Nepal. Tuberculosis 2013 Jan;93(1):84-8Download
25Malla B, Stucki D, Borrell S, Feldmann J, Maharjan B, Shrestha B, et al. First Insights into the Phylogenetic Diversity of Mycobacterium tuberculosis in Nepal. PLoS ONE 7(12): e52297. 2012Download
26Maharjan B, Shrestha B, Feldmann F, Beneke A, Pradhan DB1, Jha KK, Malla P, Akhtar M. Evaluation of GeneTypeMTBDRsl line probe assay for rapid diagnosis of XDR-TB. Int J Tubercle Lung Dis, 3rd Asia Pacific Regional Conference July 2011Download
27Maharjan B, Shrestha B, Feldmann K, Beneke A, Pradhan DB, Jha KK, Malla P, Akhatar M. Evaluation of Genotype MTBDRsl line probe assay for rapid detection of M. tuberculosis complex and it's resistant to Fluoroquinolone and aminoglycoside. Int J Tubercle Lung Dis Volume 14, Nov 2010Download
28Maharjan B, Shrestha B, Feldmann K, Beneke A, Pradhan DB, Jha KK, Malla P, Mohammad A. Evaluation of Genotype MTBDRsl for rapid détection of résistance to Floroquinolone and aminoglycosides. Int J Tubercle Lung Dis. Vol 14 Nov 2010.Download
29Konopka HA, Malomanova N, Tupasi T, Maharjan B, Cegielski P, O’Brien R. The impact of liquid culture and DST implementation in three programmatic settings. Int J Tuberc Lung Dis. Volume 13, Dec 2009.Download
30Shrestha B, Maharjan B, Feldmann K, Malla P. The identification of Mycobacterium tuberculosis complex and non Mycobacterium tuberculosis (NTM) among the patients registered in DOTS PLUS program in Nepal. Second SAARC conference, Kathmandu. Dec 08 Download
31 Urbanczik R, Shrestha B, Maharjan B, Neher A, Feldmann K. Appropriateness of using two instead of a single sputum specimen for therapy monitoring in pulmonary tuberculosis. Int J Tubercle Lung Dis 8(12):1510-1513. 2004Download

Clinic Based Publications

1 Samiksha Ghimire, Samriddhi Karki, Bhagwan Maharjan,Jos G W Kosterink, Daan J Touw, Tjip S van der Werf, Bhabana Shrestha and Jan-Willem Alffenaar. Treatment outcomes of patients with MDR-TB in Nepal on a current programmatic standardized regimen: retrospective single-centrestudy.BMJ Open Respiratory Research. 2020Download
2Malla P, Kanitz E E, Akhtar M, Falzon D, Feldmann K, Gunneberg C, Jha S.S, Maharjan B, Prasai MK, Shrestha B, Verma SC, Zignol M. Ambulatory-Based Standardized Therapy for Multi-Drug Resistant Tuberculosis: Experience from Nepal 2005–2006. PLoS ONE. 4(12), 2009Download
3Jindani A, Borgulya G, de Patiño IW, Gonzales T, de Fernandes RA, Shrestha B, Atwine D, Bonnet M, Burgos M, Dubash F, Patel N, Checkley AM, Harrison TS, Mitchison D; International Consortium for Trials of Chemotherapeutic Agents in Tuberculosis, St George's University of London. A randomized Phase II trial to evaluate the toxicity of high-dose rifampicin to treat pulmonary tuberculosis. Int J Tuberc Lung Dis. 2016
4Efficacy and safety of a 4-drug fixed-dose combination regimen compared with separate drugs for treatment of pulmonary tuberculosis: the Study C randomized controlled trial. Publication: (The journal of the American Medical Association Volume: 305 ISSN: 1538-3598 ISO Abbreviation: JAMA Publication Date: 2011 Apr)
5Yoshiyama T, Shrestha B, Maharjan B. Risk of relapse and failure after retreatment with the Cat II regimen in Nepal. Int J Tubercle Lung Dis. pp. 1418-1423(6). 2010.Download
6Two 8-month regimens of chemotherapy for treatment of newly diagnosed pulmonary tuberculosis: the Study A,international multicenter randomized trial.
Publication: The LANCET October 02-2004. Volume 364.Issue 9441 Page 1244-1251.
7A.Neher; G.Breyer; B.Shrestha; K.Feldmann. Directly observed intermittent short course chemotherapy in Kathmandu valley. Tubercle lung disease. 1996Download


  1. Genetic diversity and distribution dynamics of multidrug-resistant Mycobacterium tuberculosis isolates in Nepal
  2. Direct detection ofMycobacterium tuberculosisin clinical samples by a drymethyl green loop-mediated isothermal amplification (LAMP) method
  3. A novel sputum transport solutioneliminates cold chain and supports routinetuberculosis testing in Nepal

  4. Evaluation of OMNIgene®•SPUTUM-stabilised sputum for long-term transport and Xpert® MTB/RIF testing in Nepal